Journal: Toxics

Article Title: Factors Influencing the Formation of Chemical–Hemoglobin Adducts

doi: 10.3390/toxics10010002

Figure Lengend Snippet: Effect of combined exposure to chemicals on Hb adduct formation (in vitro). ( A ) Effect of combined exposure to acrylamide on glycidol–Hb adduct formation. * The significance level was p < 0.05 compared with 0-mM acrylamide. ( B ) Relative value of acrylamide–Hb adduct formation with combined exposure of glycidol. * The significance level was p < 0.05 compared with the acrylamide alone at each concentration. ( C ) Effect of combined exposure to glycidol on acrylamide–Hb adduct formation. The significance level was p < 0.05 compared with 0-mM glycidol. ( D ) Relative value of glycidol–Hb adduct formation with combined exposure of acrylamide. * The significance level was p < 0.05 compared with glycidol alone at each concentration. The values represent the mean ± standard deviation (SD) of four independent experiments.

Article Snippet: Acrylamide (purity 95.0%) was obtained from FUJIFILM Wako Pure Chemical Industries Ltd. (Osaka, Japan).

Techniques: In Vitro, Concentration Assay, Standard Deviation

Journal: Toxics

Article Title: Factors Influencing the Formation of Chemical–Hemoglobin Adducts

doi: 10.3390/toxics10010002

Figure Lengend Snippet: Hemoglobin (Hb) adduct formation in vivo in mice exposed to a combination of glycidol and acrylamide (in vivo). ( A ) Glycidol–Hb adduct formation following administration of glycidol alone to mice. ( B ) Acrylamide–Hb adduct formation following administration of acrylamide alone to mice. ( C ) Glycidol–Hb adduct formation in mice exposed to a combination of glycidol and acrylamide. ( D ) Acrylamide–Hb adduct formation in mice exposed to a combination of glycidol and acrylamide. * The significance level was p < 0.05 compared with the glycidol alone at each concentration. The values represent the mean ± standard deviation (SD) of four independent experiments.

Article Snippet: Acrylamide (purity 95.0%) was obtained from FUJIFILM Wako Pure Chemical Industries Ltd. (Osaka, Japan).

Techniques: In Vivo, Concentration Assay, Standard Deviation

Journal: Toxics

Article Title: Factors Influencing the Formation of Chemical–Hemoglobin Adducts

doi: 10.3390/toxics10010002

Figure Lengend Snippet: Hb adduct formation in blood from mice with diabetes mellitus (DM) reacted with glycidol or acrylamide (ex vivo). ( A ) Glycidol–Hb adduct formation in blood from the type I DM model mice. ( B ) Acrylamide–Hb adduct formation in blood from the type I DM model mice. * The significance level was p < 0.05 compared with the normal mouse blood. The values represent the mean ± standard deviation (SD) of four independent experiments.

Article Snippet: Acrylamide (purity 95.0%) was obtained from FUJIFILM Wako Pure Chemical Industries Ltd. (Osaka, Japan).

Techniques: Ex Vivo, Standard Deviation

Journal: Toxics

Article Title: Factors Influencing the Formation of Chemical–Hemoglobin Adducts

doi: 10.3390/toxics10010002

Figure Lengend Snippet: Melting profile of hemoglobin (Hb) with ( A ) glycidol or ( B ) acrylamide. The figures show the profile of the derivative of fluorescence emission as a function of temperature (dF/dT). Human Hb (0.125 mg/mL) and the ligand (0, 10, 100, 200, and 400 mM each for glycidol or acrylamide) were reacted at 37 °C for 3 h before analysis using the thermal shift assay.

Article Snippet: Acrylamide (purity 95.0%) was obtained from FUJIFILM Wako Pure Chemical Industries Ltd. (Osaka, Japan).

Techniques: Fluorescence, Thermal Shift Assay

Journal: Toxics

Article Title: Factors Influencing the Formation of Chemical–Hemoglobin Adducts

doi: 10.3390/toxics10010002

Figure Lengend Snippet: Melting temperature of hemoglobin exposed to glycidol or acrylamide.

Article Snippet: Acrylamide (purity 95.0%) was obtained from FUJIFILM Wako Pure Chemical Industries Ltd. (Osaka, Japan).

Techniques: Control

Journal: Toxics

Article Title: Factors Influencing the Formation of Chemical–Hemoglobin Adducts

doi: 10.3390/toxics10010002

Figure Lengend Snippet: Melting profiles of hemoglobin (Hb) in the presence of glucose and ( A ) glycidol or ( B ) acrylamide. The figures show the profile of the derivative of fluorescence emission as a function of temperature (dF/dT). Glycidol or acrylamide was added after the preincubation of Hb and glucose.

Article Snippet: Acrylamide (purity 95.0%) was obtained from FUJIFILM Wako Pure Chemical Industries Ltd. (Osaka, Japan).

Techniques: Fluorescence

Journal: Toxics

Article Title: Factors Influencing the Formation of Chemical–Hemoglobin Adducts

doi: 10.3390/toxics10010002

Figure Lengend Snippet: Melting temperature of hemoglobin in the presence of glucose and glycidol or acrylamide.

Article Snippet: Acrylamide (purity 95.0%) was obtained from FUJIFILM Wako Pure Chemical Industries Ltd. (Osaka, Japan).

Techniques: Control

Journal: Toxics

Article Title: Factors Influencing the Formation of Chemical–Hemoglobin Adducts

doi: 10.3390/toxics10010002

Figure Lengend Snippet: Melting profiles of hemoglobin (Hb) during combined exposure to glycidol and acrylamide. The figure shows the profile of the derivative of fluorescence emission as a function of temperature (dF/dT). Human Hb (0.125 mg/mL) and the ligand (0, 100, and 200 mM each for glycidol and acrylamide) were reacted at 37 °C for 3 h before analysis using the thermal shift assay.

Article Snippet: Acrylamide (purity 95.0%) was obtained from FUJIFILM Wako Pure Chemical Industries Ltd. (Osaka, Japan).

Techniques: Fluorescence, Thermal Shift Assay

Journal: Toxics

Article Title: Factors Influencing the Formation of Chemical–Hemoglobin Adducts

doi: 10.3390/toxics10010002

Figure Lengend Snippet: Melting temperature of hemoglobin with glycidol and acrylamide.

Article Snippet: Acrylamide (purity 95.0%) was obtained from FUJIFILM Wako Pure Chemical Industries Ltd. (Osaka, Japan).

Techniques: Control